中文Ikhithi yokuthola igciwane lesifo sohudo lwe-Porcine Epidemic RT-PCR
Igama Lomkhiqizo
I-Porcine Epidemic Diarrhea Virus RT-PCR Detection Kit (Lyophilized)
Usayizi
48tests/kit, 50tests/kit
Ukusetshenziswa Okuhlosiwe
Le kit isebenzisa indlela ye-RT-PCR ye-fluorescent yesikhathi sangempela ukuthola i-RNA ye-Porcine epidemic diarrhea virus (PEDV) ezintweni ezithinta izifo zezicubu ezifana namathoni, ama-lymph nodes kanye ne-spleen kanye nezinto eziwuketshezi zezifo ezifana nomgomo negazi lezingulube.Ilungele ukutholwa, ukuxilongwa kanye nophenyo lwe-epidemiological ye-Porcine epidemic virus yohudo.Ikhithi UHLELO LWE-PCR OLULUNGELE LONKE(Lyophilized), oluqukethe i-reverse transcriptase, i-enzyme yokukhulisa i-DNA, isibada sokusabela, ama-primers athile kanye nama-probe adingekayo ukuze kutholwe i-RT-PCR ye-fluorescent.
Okuqukethwe Komkhiqizo
| Izingxenye | Iphakheji | ukucaciswa | Isithako |
| I-PEDV PCR Mix | 1 × ibhodlela (Lyophilized powder) | 50Isivivinyo | dNTPs, MgCl2, Iziqalisi, Ama-Probes, i-Taq DNA polymerase |
| I-6 × 0.2ml 8 ithubhu ye-strip kahle(I-Lyophilized) | 48Isivivinyo | ||
| Ukulawula Okuhle | 1 * 0.2ml ishubhu (lyophilized) | 10Izivivinyo | I-Plasmid noma i-Pseudovirus equkethe izingcezu ezithile ze-PEDV |
| Isixazululo sokuqedwa | 1.5 ml Cryotube | 500uL | / |
| Ukulawula Okungalungile | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Isitoreji Nempilo Yeshelufu
(1)Ikhithi ingathuthwa ngezinga lokushisa elilingana negumbi.
(2) Impilo yeshelufu yizinyanga eziyi-18 ku -20 ℃ kanye nezinyanga eziyi-12 ku-2 ℃ ~ 30 ℃.
(3)Bona ilebula kukhithi yedethi yokukhiqiza kanye nosuku lokuphelelwa yisikhathi.
(4)I-lyophilized powder version reagent kufanele igcinwe ku--20 ℃ ngemva kokuchithwa kanye nokuqhwaza okuphindaphindiwe -kuncibilika kufanele kube izikhathi ezingaphansi kwezi-4.
Izinsimbi
I-GENECHECKER UF-150, ithuluzi le-PCR le-UF-300 lesikhathi sangempela se-fluorescence.
Umdwebo Wokusebenza
a) Inguqulo yebhodlela:
b) 8 inguqulo ye-well-strip tube:
I-Pcr Amplification
Isilungiselelo Esinconyiwe
| Isinyathelo | Umjikelezo | Izinga lokushisa (℃) | Isikhathi | Isiteshi se-Fluorescence |
| 1 | 1 | 48 | 8 imiz | / |
| 2 | 1 | 95 | 2 imiz | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Qoqa i-FAM fluorescence |
*Qaphela: Amasiginali wamashaneli e-FAM fluorescence azoqoqwa ku-60℃.
Ukutolika Imiphumela Yokuhlolwa
| Isiteshi | Ukuhunyushwa kwemiphumela |
| Isiteshi se-FAM | |
| Ct≤35 | I-PEDV Positive |
| Undet | I-PEDV Negative |
| 35 | Umphumela osolisayo, phinda uhlole* |
*Uma umphumela wokuhlolwa kabusha wesiteshi se-FAM unenani elingu-Ct ≤40 futhi ubonisa ijika lokukhulisa umumo elithi “S” elivamile, umphumela uhunyushwa njengophozithivu, ngaphandle kwalokho uthi unegethivu.
