中文I-E.coli O157:Ikhithi yokuthola i-PCR ye-H7
Igama Lomkhiqizo
I-E.coli O157:Ikhithi yokuthola i-PCR ye-H7 (I-Lyophilized)
Usayizi
48tests/kit, 50tests/kit
Ukusetshenziswa Okuhlosiwe
I-Escherichia coli O157:H7 (E.coli O157:H7) iyibhaktheriya ene-gram-negative eyingxenye yohlobo lwe-Enterobacteriaceae, ekhiqiza inani elikhulu le-Vero toxin.Ngokomtholampilo, kuvame ukuvela ngokuzumayo kube nobuhlungu obukhulu besisu kanye nesifo sohudo esimanzi, kulandele isifo sohudo esopha ngemva kwezinsuku ezimbalwa, okungaholela ekubeni nomkhuhlane noma ukungabikho umkhuhlane, nokufa ezimweni ezimbi kakhulu.Le khithi ifanele ukutholwa kwekhwalithi ye-Escherichia coli O157:H7 ekudleni, kumasampula amanzi, endle, emhlanzweni, oketshezini oluthuthukisa amagciwane namanye amasampula kusetshenziswa umgomo we-PCR yesikhathi sangempela. I-Lyophilized), equkethe i-enzyme yokukhulisa i-DNA, isivaluli sokusabela, iziqalo ezithile kanye nama-probe adingekayo ukuze kutholwe i-PCR ekhanyayo.
Okuqukethwe Komkhiqizo
| Izingxenye | Iphakheji | ukucaciswa | Isithako |
| I-E.coli O157:H7 PCR Mix | 1 × ibhodlela (Lyophilized powder) | 50Isivivinyo | dNTPs, MgCl2, Iziqalisi, Ama-Probes, Reverse Transcriptase, Taq DNA polymerase |
| I-6 × 0.2ml 8 ithubhu ye-strip kahle(I-Lyophilized) | 48Isivivinyo | ||
| Ukulawula Okuhle | 1 * 0.2ml ishubhu (lyophilized) | 10Izivivinyo | I-Plasmid equkethe i-E.coli O157:H7 izingcezu ezithile |
| Isixazululo sokuqedwa | 1.5 ml Cryotube | 500uL | / |
| Ukulawula Okungalungile | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Isitoreji Nempilo Yeshelufu
(1)Ikhithi ingathuthwa ngezinga lokushisa elilingana negumbi.
(2) Impilo yeshelufu yizinyanga eziyi-18 ku -20 ℃ kanye nezinyanga eziyi-12 ku-2 ℃ ~ 30 ℃.
(3)Bona ilebula kukhithi yedethi yokukhiqiza kanye nosuku lokuphelelwa yisikhathi.
(4)I-lyophilized powder version reagent kufanele igcinwe ku--20 ℃ ngemva kokuchithwa kanye nokuqhwaza okuphindaphindiwe -kuncibilika kufanele kube izikhathi ezingaphansi kwezi-4.
Izinsimbi
I-GENECHECKER UF-150, ithuluzi le-PCR le-UF-300 lesikhathi sangempela se-fluorescence.
Umdwebo Wokusebenza
a) Inguqulo yebhodlela:
b) 8 inguqulo ye-well-strip tube:
I-Pcr Amplification
KunconyiweUkusetha
| Isinyathelo | Umjikelezo | Izinga lokushisa (℃) | Isikhathi | Isiteshi se-Fluorescence |
| 1 | 1 | 95 | 2 imiz | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Qoqa i-FAM fluorescence |
*Qaphela: Isiginali yesiteshi se-FAM fluorescence izoqoqwa ku-60℃.
Ukutolika Imiphumela Yokuhlolwa
| Isiteshi | Ukuhunyushwa kwemiphumela |
| Isiteshi se-FAM | |
| Ct≤35 | I-E.coli O157:H7 Inhle |
| Undet | I-E.coli O157:H7 Ayinayo |
| 35 | Ukuqalwa kabusha okusolisayo, phinda uhlole* |
*Uma umphumela wokuhlolwa kabusha wesiteshi se-FAM unenani elingu-Ct ≤40 futhi ubonisa ijika lokukhulisa umumo elithi “S” elivamile, umphumela uhunyushwa njengophozithivu, ngaphandle kwalokho uthi unegethivu.
