E.coli O157: Ikhithi yokuthola i-H7 PCR

Incazelo emfushane:

I-Escherichia coli O157: H7 (E.coli O157: H7) yigciwane eline-gram-negative lohlobo lwe-Enterobacteriaceae, elikhiqiza inani elikhulu le-Vero toxin.


Imininingwane yomkhiqizo

Amathegi womkhiqizo

Igama lomkhiqizo

I-E.coli O157: Ikhithi yokuthola i-H7 PCR (Lyophilized)

Usayizi

Izivivinyo ezingama-48 / ikhithi, ama-50tests / kit

Ukusetshenziswa okuhlosiwe

I-Escherichia coli O157: H7 (E.coli O157: H7) yigciwane eline-gram-negative lohlobo lwe-Enterobacteriaceae, elikhiqiza inani elikhulu le-Vero toxin. Ngokwasemtholampilo, kuvame ukuvela ngokungazelelwe ngobuhlungu besisu obunzima kanye nohudo olunamanzi, kulandelwe isifo sohudo esibuhlungu ezinsukwini ezimbalwa kamuva, okungaholela ekuphathweni imfiva noma kungabikho mkhuhlane, nasekufeni ezimeni ezinzima. Le khithi ilungele ukutholwa kwekhwalithi ye-Escherichia coli O157: H7 ekudleni, amasampula wamanzi, indle, ukugabha, uketshezi olwandisa ibhaktheriya namanye amasampula kusetshenziswa isimiso se-PCR yesikhathi sangempela. I-Lyophilized), equkethe i-enzyme yokukhulisa i-DNA, i-reaction buffer, ama-primers athile nama-probes adingekayo ukuthola ukutholwa kwe-PCR ye-fluorescent ..

Okuqukethwe komkhiqizo

Izingxenye Iphakheji incazelo Isithako
E.coli O157: H7 PCR Mix 1 × ibhodlela (i-Lyophilized powder)  50Ukuhlolwa Ama-dNTPs, ama-MgCl2, AmaPrimers, Ama-Probes, Reverse Transcriptase, iTaq DNA polymerase
I-6 × 0.2ml 8 ithubhu yomugqa omuhle(AbakwaLyophilized) 48Ukuhlolwa
Ukulawula Okuhle 1 * 0.2ml tube (i-lyophilized)  10Uvivinyo

I-Plasmid equkethe u-E. Coli O157: izingcezu ezithile ze-H7

Ukuqedwa kwesisombululo 1.5 ml uCryotube 500uL /
Ukulawulwa Okubi 1.5 ml uCryotube 200uL 0.9% i-NaCl

Isitoreji & Impilo Yesheluli

(1) Ikhithi ingahanjiswa ekamelweni lokushisa.

(2) Impilo yeshalofu izinyanga eziyi-18 ngo -20-nezinyanga eziyi-12 ku-2 ℃ ~ 30 ℃.

(3) Bona ilebula elikukhithi yedethi yokukhiqiza nosuku lokuphelelwa yisikhathi.

(4) I-reagent yenguqulo ye-lyophilized powder kufanele igcinwe ku -20 ℃ ngemuva kokuqedwa kanye neqhwa eliphindiwe -thaw kufanele libe ngaphansi kwezikhathi ze-4.

Izinsimbi

I-GENECHECKER UF-150, i-UF-300 real-time fluorescence PCR instrument.

Umdwebo Wokusebenza

a) Uhlobo lwebhodlela:

1

b) Uhlobo lweshubhu olu-strip olu-8 kahle:

2

Ukukhuliswa kwe-Pcr

Kunconyiwe Ukusetha

Isinyathelo     Umjikelezo Ithempelesha (℃)   Isikhathi Isiteshi se-Fluorescence
1 1 95 2min  
2 40 95 5s  
60 10s Qoqa i-FAM fluorescence

* Qaphela: Isiginali yesiteshi se-FAM fluorescence sizoqoqwa ngo-60 ℃.

Ukuhumusha Imiphumela Yokuhlola

Isiteshi

Ukuhunyushwa kwemiphumela

Isiteshi se-FAM

Ct≤35

U-E.coli O157: H7 Uyaphila

Undet

E.coli O157: H7 Okubi

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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