Ikhithi yokuthola i-Pseudorabies virus (gB) PCR

Incazelo emfushane:

Le kit isebenzisa indlela ye-PCR ye-fluorescent yesikhathi sangempela ukuze ithole i-RNA ye-Pseudorabies virus (gB gene) (PRV) ezintweni ezithinta izifo zezicubu ezifana namathoni, ama-lymph nodes kanye ne-spleen kanye nezinto eziwuketshezi zezifo ezifana nomgomo negazi lezingulube.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama Lomkhiqizo

I-Pseudorabies virus (gB) PCR Detection Kit (Lyophilized)

Usayizi

48tests/kit, 50tests/kit

Ukusetshenziswa Okuhlosiwe

Le kit isebenzisa indlela ye-PCR ye-fluorescent yesikhathi sangempela ukuze ithole i-RNA ye-Pseudorabies virus (gB gene) (PRV) ezintweni ezithinta izifo zezicubu ezifana namathoni, ama-lymph nodes kanye ne-spleen kanye nezinto eziwuketshezi zezifo ezifana nomgomo negazi lezingulube.Ilungele ukutholwa, ukuxilongwa kanye nophenyo lwe-epidemiological ye-Pseudorabies virus (i-gB gene).Ikhithi UHLELO LWE-PCR LONKE-LULUNGELE (Lyophilized), oluqukethe i-enzyme yokukhulisa i-DNA, isivaluli sokusabela, iziqalo ezithile kanye nama-probe adingekayo ukuze kutholwe i-RT-PCR yefluorescent.

Okuqukethwe Komkhiqizo

Izingxenye Iphakheji ukucaciswa Isithako
I-PRV PCR Mix 1 × ibhodlela (Lyophilized powder)  50Isivivinyo dNTPs, MgCl2, Iziqalisi, Ama-Probes, i-Taq DNA polymerase
I-6 × 0.2ml 8 ithubhu ye-strip kahle(I-Lyophilized) 48Isivivinyo
Ukulawula Okuhle 1 * 0.2ml ishubhu (lyophilized)  10Izivivinyo

I-Plasmid noma i-Pseudovirus equkethe izingcezu ezithile ze-PRV(gB).

Isixazululo sokuqedwa 1.5 ml Cryotube 500uL /
Ukulawula Okungalungile 1.5 ml Cryotube 200uL 0.9%NaCl

Isitoreji Nempilo Yeshelufu

(1)Ikhithi ingathuthwa ngezinga lokushisa elilingana negumbi.

(2) Impilo yeshelufu yizinyanga eziyi-18 ku -20 ℃ kanye nezinyanga eziyi-12 ku-2 ℃ ~ 30 ℃.

(3)Bona ilebula kukhithi yedethi yokukhiqiza kanye nosuku lokuphelelwa yisikhathi.

(4)I-lyophilized powder version reagent kufanele igcinwe ku--20 ℃ ngemva kokuchithwa kanye nokuqhwaza okuphindaphindiwe -kuncibilika kufanele kube izikhathi ezingaphansi kwezi-4.

Izinsimbi

I-GENECHECKER UF-150, ithuluzi le-PCR le-UF-300 lesikhathi sangempela se-fluorescence.

Umdwebo Wokusebenza

a) Inguqulo yebhodlela:

1

b) 8 inguqulo ye-well-strip tube:

2

I-Pcr Amplification

Isilungiselelo Esinconyiwe

Isinyathelo

Umjikelezo

Izinga lokushisa (℃)

Isikhathi

Isiteshi se-Fluorescence

1

1

95

2 imiz

/

2

40

95

5s

/

60

10s

Qoqa i-FAM fluorescence

*Qaphela: Amasiginali wamashaneli e-FAM fluorescence azoqoqwa ku-60℃.

Ukutolika Imiphumela Yokuhlolwa

Isiteshi

Ukuhunyushwa kwemiphumela

Isiteshi se-FAM

Ct≤35

I-PRV(gB) Ilungile

Undet

I-PRV(gB) Inegethivu

35

Ukuqalwa kabusha okusolisayo, phinda uhlole*

*Uma umphumela wokuhlolwa kabusha wesiteshi se-FAM unenani elingu-Ct ≤40 futhi ubonisa ijika lokukhulisa umumo elithi “S” elivamile, umphumela uhunyushwa njengophozithivu, ngaphandle kwalokho uthi unegethivu.


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