Ikhithi Yokuthola I-Salmonella PCR

Incazelo emfushane:

I-Salmonella iyingxenye ye-Enterobacteriaceae ne-Gram-negative enterobacteria.I-Salmonella iyi-pathogen evamile etholakala ekudleni futhi iqala kuqala ebusheni bokudla kwamagciwane.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama Lomkhiqizo

I-Salmonella PCR Detection Kit (i-lyophilized)

Usayizi

48tests/kit, 50tests/kit

Ukusetshenziswa Okuhlosiwe

I-Salmonella iyingxenye ye-Enterobacteriaceae ne-Gram-negative enterobacteria.I-Salmonella iyi-pathogen evamile etholakala ekudleni futhi iqala kuqala ebusheni bokudla kwamagciwane.Izimpawu eziyinhloko zobuthi bokudla obubangelwa i-Salmonella isicanucanu, ukuhlanza, ubuhlungu besisu, ikhanda elibuhlungu, ukugodola kanye nesifo sohudo.Le khithi isebenzisa umgomo we-PCR ye-fluorescent yesikhathi sangempela futhi ifanele ukutholwa kwekhwalithi ye-Salmonella ekudleni, amasampula amanzi, indle, ukuhlanza, noketshezi olunothisayo.

Okuqukethwe Komkhiqizo

Izingxenye	Iphakheji	ukucaciswa	Isithako
I-PCR Mix	1 × ibhodlela (Lyophilized powder)	50Isivivinyo	dNTPs, MgCl₂, Iziqalo, Ama-Probes, i-Taq DNA polymerase
I-PCR Mix	I-6 × 0.2ml 8 ithubhu ye-strip kahle(I-Lyophilized)	48Isivivinyo	dNTPs, MgCl₂, Iziqalo, Ama-Probes, i-Taq DNA polymerase
Ukulawula Okuhle	1 * 0.2ml ishubhu (lyophilized)	10Izivivinyo	I-Pseudovirus noma i-Pseudovirus equkethe izingcezu ezithile
Isixazululo sokuqedwa	1.5 ml Cryotube	500uL	/
Ukulawula Okungalungile	1.5 ml Cryotube	100uL	0.9%NaCl

Isitoreji Nempilo Yeshelufu

(1)Ikhithi ingathuthwa ngezinga lokushisa elilingana negumbi.

(2) Impilo yeshelufu yizinyanga eziyi-18 ku -20 ℃ kanye nezinyanga eziyi-12 ku-2 ℃ ~ 30 ℃.

(3)Bona ilebula kukhithi yedethi yokukhiqiza kanye nosuku lokuphelelwa yisikhathi.

(4)I-reagent ye-lyophilized powder version kufanele igcinwe ku--20 ℃ ngemva kokuchithwa kanye nokuqhwaza okuphindaphindiwe -kuncibilika kufanele kube izikhathi ezingaphansi kwezi-4.

Izinsimbi

I-GENECHECKER UF-150, ithuluzi le-PCR le-UF-300 lesikhathi sangempela se-fluorescence.

Umdwebo Wokusebenza

a) Inguqulo yebhodlela:

b) 8 inguqulo ye-well-strip tube:

I-Pcr Amplification

Isilungiselelo Esinconyiwe

Isinyathelo	Umjikelezo	Izinga lokushisa (℃)	Isikhathi	Isiteshi se-Fluorescence
1	1	95	2 imiz	/
2	40	95	5s	/
2	40	60	10s	Qoqa i-FAM fluorescence

Ukutolika Imiphumela Yokuhlolwa

Isiteshi	Ukuhunyushwa kwemiphumela
Isiteshi se-FAM	Ukuhunyushwa kwemiphumela
Ct≤35	I-Salmonella Positive
Undet	I-Salmonella Negative
35	Ukuqalwa kabusha okusolisayo, phinda uhlole*

*Uma umphumela wokuhlolwa kabusha wesiteshi se-FAM unenani elingu-Ct ≤40 futhi ubonisa ijika elivamile lokukhulisa umumo “S”, umphumela uhunyushwa ngokuthi phozithivu, ngaphandle kwalokho uthi unegethivu.

Okwedlule: I-Norovirus (GⅡ) RT-PCR Detection Kit

Olandelayo: Ikhithi Yokuthola i-Shigella PCR