中文I-Vibrio parahaemolyticus PCR Detection Kit
Igama Lomkhiqizo
I-Vibrio Parahaemolyticus PCR Detection Kit (Lyophilized)
Usayizi
48tests/kit, 50tests/kit
Ukusetshenziswa Okuhlosiwe
I-Vibrio Parahemolyticus (eyaziwa nangokuthi i-Halophile Vibrio Parahemolyticus) iyi-Gram-negative polymorphic bacillus noma i-Vibrio Parahemolyticus.eqala kakhulu, ubuhlungu besisu, ukuhlanza, isifo sohudo kanye nendle enamanzi njengezimpawu zomtholampilo eziyinhloko.Le kit isebenzisa isimiso se-fluorescent yesikhathi sangempela I-PCR futhi ifanele ukutholwa kwekhwalithi ye-Vibrio Parahaemolyticus ekudleni, kumasampula amanzi, endle, ekuhlanzeni, nakuketshezi olunothisayo.Ikhithi YI-ALL-READY PCR SYSTEM(Lyophilized), equkethe i-enzyme yokukhulisa i-DNA, i-react buffer, iziqalo ezithile. kanye nama-probe adingekayo ukuze kutholwe i-fluorescent RT-PCR.
Okuqukethwe Komkhiqizo
| Izingxenye | Iphakheji | ukucaciswa | Isithako |
| I-PCR Mix | 1 × ibhodlela (Lyophilized powder) | 50Isivivinyo | dNTPs, MgCl2, Iziqalisi, Ama-Probes, i-Taq DNA polymerase |
| I-6 × 0.2ml 8 ithubhu ye-strip kahle(I-Lyophilized) | 48Isivivinyo | ||
| Ukulawula Okuhle | 1 * 0.2ml ishubhu (lyophilized) | 10Izivivinyo | I-Pseudovirus noma i-Pseudovirus equkethe izingcezu ezithile |
| Isixazululo sokuqedwa | 1.5 ml Cryotube | 500uL | / |
| Ukulawula Okungalungile | 1.5 ml Cryotube | 100uL | 0.9%NaCl |
Isitoreji Nempilo Yeshelufu
(1)Ikhithi ingathuthwa ngezinga lokushisa elilingana negumbi.
(2) Impilo yeshelufu yizinyanga eziyi-18 ku -20 ℃ kanye nezinyanga eziyi-12 ku-2 ℃ ~ 30 ℃.
(3)Bona ilebula kukhithi yedethi yokukhiqiza kanye nosuku lokuphelelwa yisikhathi.
(4)I-lyophilized powder version reagent kufanele igcinwe ku--20 ℃ ngemva kokuchithwa kanye nokuqhwaza okuphindaphindiwe -kuncibilika kufanele kube izikhathi ezingaphansi kwezi-4.
Izinsimbi
I-GENECHECKER UF-150, ithuluzi le-PCR le-UF-300 lesikhathi sangempela se-fluorescence.
Umdwebo Wokusebenza
a) Inguqulo yebhodlela:
b) 8 inguqulo ye-well-strip tube:
I-Pcr Amplification
Isilungiselelo Esinconyiwe
| Isinyathelo | Umjikelezo | Izinga lokushisa (℃) | Isikhathi | Isiteshi se-Fluorescence |
| 1 | 1 | 95 | 2 imiz | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Qoqa i-FAM fluorescence |
Ukutolika Imiphumela Yokuhlolwa
| Isiteshi | Ukuhunyushwa kwemiphumela |
| Isiteshi se-FAM | |
| Ct≤35 | Vibrio Parahaemolyticus Positive |
| Undet | I-Vibrio Parahaemolyticus Negative |
| 35 | Ukuqalwa kabusha okusolisayo, phinda uhlole* |
*Uma umphumela wokuhlolwa kabusha wesiteshi se-FAM unenani elingu-Ct ≤40 futhi ubonisa ijika lokukhulisa umumo elithi “S” elivamile, umphumela uhunyushwa njengophozithivu, ngaphandle kwalokho uthi unegethivu.
