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I-Listeria monocytogenes PCR Detection Kit

Ikhithi Yokuthola I-Listeria monocytogenes PCR Isithombe Esifakiwe
  • I-Listeria monocytogenes PCR Detection Kit

Incazelo emfushane:

I-Listeria monocytogenes iyi-microbacterium e-gram-positive engakhula phakathi kuka-4℃ no-45℃.Ingenye yezifo eziyinhloko ezisongela impilo yabantu ekudleni okufakwe esiqandisini.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama Lomkhiqizo

I-Listeria monocytogenes PCR Detection Kit

Usayizi

48tests/kit, 50tests/kit

Ukusetshenziswa Okuhlosiwe

I-Listeria monocytogenes iyi-microbacterium e-gram-positive engakhula phakathi kuka-4℃ no-45℃.Ingenye yezifo eziyinhloko ezisongela impilo yabantu ekudleni okufakwe esiqandisini.Ukubonakaliswa okuyinhloko kokutheleleka yi-septicemia, meningitis kanye ne-mononucleosis.Le khithi ifanele ukutholwa kwekhwalithi ye-Listeria monocytogenes ekudleni, amasampula amanzi, indle, ukuhlanza, uketshezi oluthuthukisa amagciwane namanye amasampula kusetshenziswa umgomo we-PCR ye-fluorescence yesikhathi sangempela. , equkethe i-enzyme yokukhulisa i-DNA, i-reverse transcriptase, i-reaction buffer, ama-primers athile nama-probe adingekayo ukuze kutholwe i-PCR ekhanyayo.

Isitoreji Nempilo Yeshelufu

(1)Ikhithi ingathuthwa ngezinga lokushisa elilingana negumbi.

(2) Impilo yeshelufu yizinyanga eziyi-18 ku -20 ℃ kanye nezinyanga eziyi-12 ku-2 ℃ ~ 30 ℃.

(3)Bona ilebula kukhithi yedethi yokukhiqiza kanye nosuku lokuphelelwa yisikhathi.

(4)I-lyophilized powder version reagent kufanele igcinwe ku--20 ℃ ngemva kokuchithwa kanye nokuqhwaza okuphindaphindiwe -kuncibilika kufanele kube izikhathi ezingaphansi kwezi-4.

Okuqukethwe Komkhiqizo

Izingxenye Iphakheji ukucaciswa Isithako
I-Listeria monocytogenes PCR Mix 1 × ibhodlela (Lyophilized powder)  50Isivivinyo dNTPs, MgCl2, Iziqalisi, Ama-Probes, Reverse Transcriptase, Taq DNA polymerase
I-6 × 0.2ml 8 ithubhu ye-strip kahle(I-Lyophilized) 48Isivivinyo
Ukulawula Okuhle 1 * 0.2ml ishubhu (lyophilized)  10Izivivinyo

I-Plasmid equkethe izingcezu ezithile ze-Listeria monocytogenes
Isixazululo sokuqedwa 1.5 ml Cryotube 500uL /
Ukulawula Okungalungile 1.5 ml Cryotube 200uL 0.9%NaCl

Izinsimbi

I-GENECHECKER UF-150, ithuluzi le-PCR le-UF-300 lesikhathi sangempela se-fluorescence.

Umdwebo Wokusebenza

a)

1

b)

2

I-Pcr Amplification

KunconyiweUkusetha

Isinyathelo Umjikelezo Izinga lokushisa (℃) Isikhathi Isiteshi se-Fluorescence
1 1 50 8 imiz  
2 1 95 2 imiz  
3 40 95 5s  
60 10s Qoqa i-FAM fluorescence

*Qaphela: Amasiginali wamashaneli e-FAM fluorescence azoqoqwa ku-60℃.

Ukutolika Imiphumela Yokuhlolwa

Isiteshi

Ukuhunyushwa kwemiphumela

Isiteshi se-FAM

Ct≤35

 I-Listeria monocytogenes Positive

Undet

 I-Listeria monocytogenes Negative

35

 Ukuqalwa kabusha okusolisayo, phinda uhlole*

*Uma umphumela wokuhlolwa kabusha wesiteshi se-FAM unenani elingu-Ct ≤40 futhi ubonisa ijika lokukhulisa umumo elithi “S” elivamile, umphumela uhunyushwa njengophozithivu, ngaphandle kwalokho uthi unegethivu.

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